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. 2023 Jan 4;14(1):e03121-22. doi: 10.1128/mbio.03121-22

FIG 6.

FIG 6

K. pneumoniae CPS and the T6SS are required for gut colonization. (A) CFU per gr of small intestine, cecum, and colon of mice infected with Kp52145, the isogenic cps mutant (ΔmanC), and the T6SS mutant (ΔclpV).14 to 17 mice were included in each group in two independent experiments. (B) Relative distribution of Kp52145 and the isogenic cps (ΔmanC) and T6SS (ΔclpV) mutants across the intestine sections. (C) To determine the effect of the gut microbiome on the colonization by the T6SS mutant, mice were pretreated with an antibiotic cocktail that was stopped 3 days preinfection. Three days postinfection, the bacterial burdens in tissues were determined. (D) CFU per gr of small intestine, cecum, and colon of mice infected with Kp52145 and the T6SS mutant (ΔclpV) that were pretreated or not with the antibiotic cocktail (AA). 10 to 15 mice were included in each group in two independent experiments. (E) Relative distribution of Kp52145 across the intestine sections of mice pretreated or not with the antibiotic cocktail (AA). (F) CFU per gr of small intestine, cecum, and colon of E. cloacae and Klebsiella spp that were only found in mice infected with the T6SS mutant (ΔclpV) and pretreated with the antibiotic cocktail. In all panels, each value is presented as the mean ± SD. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, P > 0.05 for the indicated comparisons, which were determined using a one way-ANOVA with the Bonferroni correction for testing multiple comparisons. In panel B, *, P ≤ 0.05; **, P ≤ 0.01; ns, P > 0.05 for the comparisons, which were determined using a one way-ANOVA with the Bonferroni correction for testing multiple comparisons, of the percentage of bacteria in each of the tissue sections between the mice infected with Kp52145 and each of the mutants. In panel C, *, P ≤ 0.05; ****, P ≤ 0.0001 for the comparisons, which were determined using a Mann-Whitney U test, of the percentage of bacteria in each of the tissue sections between the mice infected with Kp52145 and those treated or not with the antibiotic cocktail (AA).