FIG 5.

Sirt1 deficiency enhances the expression of type I IFN, ISGs, and proinflammatory cytokines. (A) Volcano plots comparing gene expression in wild-type (WT) and Sirt1-deficient (KO) PMA-THP1 cells after being stimulated with HSV-1 (MOI = 1) for 4 h. (B) Heat map depiction of differentially expressed genes in WT and KO PMA-THP1 cells. (C) GSEA analysis of cytosolic DNA sensing pathway relative to WT and KO PMA-THP1 cells. Enrichment plots are shown along with the normalized enrichment score and P value. (D) KEGG analysis through Tom (https://biosys.bgi.com/) for genes significantly upregulated (log₂ ≥ 1, P < 0.05) after stimulation with HSV-1 (MOI = 1) for 4 h. (E) Heat map view of mRNA variations in type I IFN, ISGs, and proinflammatory cytokines (log₂ ≥ 2).