FIG 6.

Sirt1 decreases IFI16 acetylation. (A) HaCaT keratinocytes were transfected with the indicated plasmids for 24 h and then transfected with HSV60 (1 μg/mL) for 12 h or left untreated. Afterward, the cells were lysed and subjected to immunoprecipitation (IP) and immunoblot (IB) analysis. (B) PMA-THP1 cells were transfected with control siRNA (SC) or Sirt1-specific siRNA (S1 and S2) for 24 h and then infected with HSV-1 (MOI = 1) for 12 h. Afterward, the cells were lysed and subjected to IP and IB analysis. (C) PMA-THP1 cells were transfected with control siRNA (SC) or Sirt1-specific siRNA (S1) for 24 h and then infected with HSV-1 (MOI = 1) for the indicated periods. Afterward, the cells were lysed and subjected to IP and IB analysis. (D) Wild-type (WT) and Sirt1-deficient (KO) PMA-THP1 cells were infected with HSV-1 (MOI = 1) for the indicated periods. The cells were subjected to IP and IB analysis. (E) PMA-THP1 cells were treated with increasing amounts of SRT2104 (0, 2, and 5 μM) for 12 h and then infected with HSV-1 (MOI = 1) for another 12 h. The cells were subjected to IP and IB analysis. (F) PMA-THP1 cells were treated with DMSO, EX527 (5 μM), or resveratrol (RSV) (100 μM) for 12 h and then infected with HSV-1 (MOI = 1) for another 12 h. The cells were subjected to IP and IB analysis. (G) HEK293T cells were transfected with the indicated plasmids. At 24 h after transfection, the cells were subjected to IP and IB analysis. (H) HaCaT keratinocytes were transfected with the indicated plasmids for 24 h and then infected with HSV-1 (MOI = 1) for 8 h. Then, the cells were lysed for real-time PCR analyses. The data are representative of three independent experiments and are presented as means ± SDs. **, P < 0.01; ***, P < 0.001.