FIG 5.
Confocal imaging of viroplasms in rRV-WT- or rRV-NSP2K294E-infected cells. MA104 cells on glass coverslips were infected with rRV-WT or rRV-NSP2K294E at an MOI of 3 PFU per cell for 4, 8, 12, 16m or 24 h. (A and B) Cells were fixed with methanol and stained using αNSP2 and an Alexa-546-conjugated secondary antibody. Nuclei were stained using Hoechst. Confocal microscopy was used to determine the localization of NSP2 (561 nm; red) and nuclei (405 nm; blue). Scale bar = 10 μm. (C and D) Cells were fixed with methanol and stained using αVP1 or αVP2 and an Alexa-546-conjugated secondary antibody. Nuclei were stained using Hoechst. Confocal microscopy was used to determine the localization of VP1 or VP2 (561 nm; red) and nuclei (405 nm; blue). Scale bar = 10 μm.