FIG 1.

Immunization with MAPS1 elicits robust Mtb antigen-specific antibody and cellular responses and protects mice against Mtb aerosol infection. (A and B) Mice (n = 10 per group) were immunized three times with adjuvant vehicle (Alum) or MAPS1 (MAPS1). Peripheral blood was taken 2 weeks after the last immunization for antibody and cellular response analysis. (A) IgG titers against each Mtb protein. Titers were expressed in arbitrary units (a.u.) compared to a standard serum for each Mtb protein. (B) IFN-γ and IL-17A levels in culture supernatants after stimulating blood cells with a mixture of six Mtb proteins (2 μg/mL). (C) Mice (n = 10 per group) were immunized three times with adjuvant alone (Alum) or MAPS1 as described above. The BCG group received one dose of BCG at the first immunization and no boosters. Two weeks after the third immunization (6 weeks after BCG immunization), mice were infected with aerosolized Mtb strain H37Rv. One month later, lungs were harvested for CFU enumeration. Bars indicate medians plus 95% confidence intervals. Lines indicate medians. Dotted lines represent the lower detection limit.