FIG 2.

Combined vaccination with MAPS2 and BCG provides enhanced protection against Mtb lung infection compared with either vaccine used alone. (A) Five groups of mice (n = 10 per group) received three immunizations with reagents indicated in the table. (B) Peripheral blood was taken 2 weeks after the last immunization and stimulated with Mtb lysate for 6 days. IFN-γ and IL-17A levels in culture supernatants were measured by ELISA. (C) Two weeks after bleeding (1 month after the last immunization), mice were infected with Mtb. One month later, lungs and spleens were harvested for CFU enumeration. Bars indicate medians plus 95% confidence intervals. Lines indicate medians. Dotted lines represent the lower detection limit.