FIG 3.

Immunization with MAPS2 induces long-lived systemic and tissue-resident T memory cells. Mice (n = 10 per group) were immunized three times with adjuvant vehicle (Alum) or MAPS2. Six months later, blood, spleens, nasal tissue, and lungs were collected, and different populations of CD4⁺ and CD8⁺ T memory cells were quantitated by flow cytometry. (A) Representative zebra plot of the frequency of CD4⁺ or CD8⁺ T_CM (CD62L⁺ CXCR3⁺ CD44^high), T_EM (CD62L⁻ CD44^high), or T_RM (CD62L⁻ CD44^high/CD69⁺) in the lung of Alum- or MAPS2-immunized mice. (B) Absolute counts of CD4⁺ or CD8⁺ T_CM, T_EM, and T_RM in 100 μL of blood, 1/80 of total splenocytes, 1/3 of total nasal cells, and 1/5 of total lung cells (including both lobes) isolated from Alum- (open circles) or MAPS2-immunized mice (gray circles). n = 5 mice per group per analysis. The data represent a summary of two individual analyses. Lines indicate medians. Dotted lines represent the lower detection limit.