Table 1.
Formulation of the different experimental diets (air-dry basis, %).
| Diets | LL | HL |
|---|---|---|
| Ingredients (% dry weight) | ||
| Fish meal | 5.00 | 5.00 |
| Soybean meal | 9.00 | 9.00 |
| Cottonseed meal | 15.00 | 15.00 |
| Rapeseed meal | 12.07 | 12.07 |
| Wheat bran | 11.59 | 11.59 |
| Wheat Middlings | 37.00 | 37.00 |
| Fish oil | 2.02 | 2.02 |
| Soybean oil | 2.02 | 2.02 |
| Premixa | 1.00 | 1.00 |
| Ca (H2PO4)2 | 1.80 | 1.80 |
| Salt | 0.50 | 0.50 |
| L-leucine | 0.00 | 0.54 |
| Glycine | 3.00 | 2.46 |
| Total L-leucine | 1.61 | 2.15 |
| Proximate composition (% dry matter) | ||
| Moisture | 10.50 | 10.60 |
| Crude protein | 31.04 | 32.69 |
| Ether extract | 6.49 | 6.67 |
| Ash | 7.97 | 7.96 |
| L-leucine | 1.72 | 2.39 |
L-leucine and Glycine purity reached 99% and were purchased from Shandong Gukang Biological Engineering Co., Ltd., Shandong, China. a Premix supplied the following minerals (g/kg of diet) and vitamins (IU or mg/kg of diet): CuSO4·5H2O, 2.0 g; FeSO4·7H2O, 25 g; ZnSO4·7H2O, 22 g; MnSO4·4H2O, 7 g; Na2SeO3, 0.04 g; KI, 0.026 g; CoCl2·6H2O, 0.1 g; Vitamin A, 900,000 IU; vitamin D, 200,000 IU; vitamin E, 4500 mg; vitamin K3, 220 mg; vitamin B1, 320 mg; vitamin B2, 1090 mg; vitamin B5, 2000 mg; vitamin B6, 500 mg; vitamin B12, 1.6 mg; vitamin C, 5000 mg; pantothenate, 1000 mg; folic acid, 165 mg; and choline, 60,000 mg.