Figure 2.

Effects of NaP on intestinal histomorphology and intestinal mucosal barrier. (a) The intestine of turbot in different groups was collected and sectioned. After the fixation by H&E, the morphology of the distal intestines was observed. The images were representative of at least three independent experiments, and scale bar indicates 500 μm; (A) and (B) in the images indicate villus height and muscle layer thickness, respectively; lamina propria is indicated by arrows. (b–d) The micromorphology, including villus height (b), lamina propria (c), and muscle layer thickness (d) of the intestine, was evaluated (n = 6). (e–g) The gene expression of ZO-1 (e), occludin (f), and claudin (g) in the intestine was analyzed by qRT-PCR (n = 12). Different superscript letters indicated significant difference (P < 0.05).