Figure 6.

Effects of NaP on the bactericidal activity and the production of antibacterial effectors in HKMs. After the feeding trial, HKMs were isolated and cultured for 24 h. (a) E. tarda was added (cells: bacteria =1 : 1) and coincubated for 2 h. The bactericidal activity of HKMs was assessed (n = 6). (b and c) The gene expression of lysozyme (b) and enzyme activity (c) in the cells were measured (n = 6). (d and e) E. tarda was added (cells: bacteria =1 : 1) and coincubated for 2 h; the production of ROS (d) and NO (e) in the cells was analyzed (n = 6). Different superscript letters indicated significant difference (P < 0.05).