Skip to main content
. Author manuscript; available in PMC: 2023 Feb 28.
Published in final edited form as: Nat Cell Biol. 2022 Feb 7;24(2):217–229. doi: 10.1038/s41556-021-00827-2

Extended Data Fig. 5. Presence of WAGO-3 in spermatozoa is dependent on the IDR of PEI-1.

Extended Data Fig. 5

a, Confocal micrographs showing spermatogenesis of late-L4 stage hermaphrodites expressing PEI 1::mTagRFP-T in indicated mutants. sc – spermatocyte, rb – residual body, st – spermatid. Images represent two biologically independent experiments. Scale bars: 10 μm. b-h, Confocal maximum intensity projections of hermaphrodite-derived spermatozoa within the spermatheca expressing indicated proteins. In all panels, except c, a piece of a gonad arm expressing GFP::3xFLAG::WAGO-3 is visible in the top part of the image. Dashed lines indicate spermatheca. Images represent two biologically independent experiments. Scale bars: 10 μm.