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. Author manuscript; available in PMC: 2023 Feb 28.
Published in final edited form as: Nat Cell Biol. 2022 Feb 7;24(2):217–229. doi: 10.1038/s41556-021-00827-2

Fig. 6 ∣. PEI granules are associated with membranous organelles.

Fig. 6 ∣

a-e, Representative dual-color CLEM images acquired in indicated germ cells of spermatogenesis (indicated at the top) of high-pressure frozen adult males expressing GFP::3xFLAG::WAGO-3 and PEI-1::mTagRFP-T. The four rows show the EM-only (a-e), EM with annotations (a’-e’), EM with GFP::3xFLAG::WAGO-3 fluorescence (a”-e”), and EM with PEI-1::mTagRFP-T fluorescence (a’”-e’”). The GFP::3xFLAG::WAGO-3-only positive focus in panel a” (black arrow head) is adjacent to the nucleus, and most likely representing a P granule. Images represent two biologically independent experiments. Annotations: EM - electron microscopy, CLEM - correlative light and electron microscopy, N – nucleus, M – mitochondrion, MO – membranous organelle, FB – fibrous body, GO – Golgi complex, NH – peri-nuclear halo, D – DNA. Scale bars: 500 nm. Source data are provided.