FIG 4.

eetB genes frequently colocalize with ApbE-associated extracytosolic flavinylation system genes in bacterial genomes. (A) Pie chart showing the number of GTDB reference genomes/phylum that contain an eetB homolog (see Data Set S1 in the supplemental material for additional context). (B) Proposed basis of flavin export for L. monocytogenes flavinylation and EET activity. DHNA and DMK refer to 1,4-dihydroxy-2- naphthoate and demethylmenaquinone, respectively. The pie chart shows the number of GTDB reference genomes that contain a gene cluster with both EET and eetB genes. (C) Proposed models of flavin export in previously identified flavinylation-associated MsrQ-like, NapH, and Rnf complexes and speculative electron transfer pathways (4). The pie charts show the number of GTDB reference genomes that contain a system gene cluster with an eetB homolog.