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. 2023 Jan 17;14(1):e03051-22. doi: 10.1128/mbio.03051-22

TABLE 1.

Exclusive unique peptide contenta

No. Identified protein Accession no. Live_1 Live_2 HI_1 HI_2 None_1 None_2 OMVs
1 Aldehyde-alcohol dehydrogenase (adhE) ADHE_ECO57 58 55 0 0 0 0 +
2 Outer membrane protein A (ompA) OMPA_ECO57 32 45 3 2 0 0 +
3 60-kDa chaperonin 1 (groL1) CH601_ECOK1 28 30 0 0 0 0 +
4 Periplasmic serine endoprotease DegP (degP) DEGP_ECO57 22 14 0 0 0 0 +
5 Fe3+ dicitrate transport protein FecA (fecA) FECA_ECOLI 20 13 0 0 0 0 +
6 Colicin I receptor (car) CIRA_ECOLI 20 26 0 1 0 0 +
7 Outer membrane protein TolC (tolC) TOLC_ECOLI 20 17 0 1 0 0 +
8 Uncharacterized protein YncE (yncE) YNCE_ECOLI 19 16 0 0 0 0 +
9 Ferrienterobactin receptor (fepA) FEPA_ECOLI 18 19 0 0 0 0 +
10 Vitamin B12 transporter BtuB (btuB) BTUB_ECOLI 17 8 0 0 0 0 +
11 30S ribosomal protein S4 (rpsD) RS4_ECO24 17 17 0 0 0 0 +
12 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (aceF) ODP2_ECOLI 15 11 0 0 0 0 +
13 30S ribosomal protein S7 (rpsG) RS7_ECOBW 15 13 0 0 0 0 +
14 DNA-directed RNA polymerase subunit beta′ (rpoC) RPOC_ECO24 14 16 1 0 0 0 +
15 Outer membrane protease OmpP (ompP) OMPP_ECOLI 14 6 0 0 0 0 +
16 Protein TolB (tolB) TOLB_ECO24 13 9 0 0 0 0 +
17 Elongation factor Tu 1 (tuf1) EFTU1_ECO24 13 11 0 0 0 0 +
18 Outer membrane protein X (ompX) OMPX_ECO57 13 9 0 1 0 0 +
19 Metal-binding protein ZinT (zinT) ZINT_ECOLI 12 9 0 0 0 0
20 Major outer membrane lipoprotein (lpp) LPP_ECO57 12 15 1 0 0 0 +
21 Cell division coordinator CpoB (cpoB) CPOB_ECOLI 11 6 0 0 0 0
22 High-affinity zinc uptake system protein ZnuA (znuA) ZNUA_ECOLI 10 10 0 0 0 0 +
23 Type 1 fimbrial protein, A chain (fimA) FIMA1_ECOLI 10 12 3 4 0 0
24 Outer membrane protein assembly factor BamA (bamA) BAMA_ECO24 8 9 0 0 0 0
25 Glyceraldehyde-3-phosphate dehydrogenase A (gapA) G3P1_ECO57 8 4 0 0 0 0
26 30S ribosomal protein S13 (rpsM) RS13_ECO57 8 9 0 0 0 0 +
27 Ribose import binding protein RbsB (rbsB) RBSB_ECOLI 8 6 0 0 0 0 +
28 50S ribosomal protein L17 (rplQ) RL17_ECO24 7 8 0 0 0 0 +
29 FKBP-type peptidyl-prolyl cis-trans isomerase FkpA (fkpA) FKBA_ECO57 7 8 0 0 0 0
30 Putative acyl-CoA thioester hydrolase YbhC (ybhC) YBHC_ECOLI 7 5 0 0 0 0 +
31 Chaperone protein Skp (skp) SKP_ECO57 7 3 0 0 0 0 +
32 Outer membrane lipoprotein SlyB (slyB) SLYB_ECO57 7 4 0 0 0 0 +
33 50S ribosomal protein L21 (rplU) RL21_ECO24 7 3 0 0 0 0
34 DNA-directed RNA polymerase subunit alpha (rpoA) RPOA_ECO24 6 11 0 1 0 0
35 Peptidoglycan-associated lipoprotein (pal) PAL_ECO57 6 7 0 0 0 0 +
36 Phosphate-binding protein PstS (pstS) PSTS_ECOLI 6 3 0 0 0 0
37 50S ribosomal protein L4 (rplD) RL4_ECO24 6 8 0 0 0 0
38 Outer membrane protein assembly factor BamC (bamC) BAMC_ECOLI 6 5 0 0 0 0
39 Uncharacterized protein YegR (yegR) YEGR_ECOLI 6 2 0 0 0 0 +
40 Protein FimH (fimH) FIMH_ECOLI 6 4 0 0 0 0
41 50S ribosomal protein L20 (rplT) RL20_ECO24 5 10 0 0 0 0 +
42 Uncharacterized protein YqjD (yqjD) YQJD_ECO57 5 3 0 0 0 0 +
43 Outer membrane protein assembly factor BamB (bamB) BAMB_ECOLI 5 6 0 0 0 0
44 50S ribosomal protein L24 (rplX) RL24_ECO24 5 3 0 0 0 0
45 50S ribosomal protein L2 (rplB) RL2_ECO24 4 6 0 0 0 0 +
46 50S ribosomal protein L1 (rplA) RL1_ECO27 4 7 0 0 0 0 +
47 Uncharacterized lipoprotein YajG (yajG) YAJG_ECOL6 4 8 0 0 0 0 +
48 Penicillin-binding protein activator LpoA (lpoA) LPOA_ECOLI 4 5 0 3 0 0
49 Rare lipoprotein A (rlpA) RLPA_ECOLI 4 3 0 0 0 0 +
50 Protein YdgH (ydgH) YDGH_ECOLI 4 2 0 0 0 0 +
51 50S ribosomal protein L15 (rplO) RL15_ECO24 4 4 0 0 0 0
52 30S ribosomal protein S6 (rpsF) RS6_ECO24 4 7 0 0 0 0
53 Outer membrane protein (ompC) OMPC_ECOLI 3 7 0 0 0 0 +
54 Protein TraN GN (traN) TRAN_ECOLI 3 2 0 0 0 0
55 Chaperone protein FimC (fimC) FIMC_ECOL6 3 2 0 0 0 0
56 30S ribosomal protein S9 (rpsI) RS9_ECO24 3 6 0 0 0 0 +
57 LPS assembly protein LptD (lptD) LPTD_ECOK1 3 3 0 0 0 0
58 TraT complement resistance protein (traT) TRAT1_ECOLI 3 6 0 0 0 0
59 Nucleoside-specific channel-forming protein tsx (tsx) TSX_ECO57 3 4 0 0 0 0 +
60 Ferrichrome-iron receptor (fhuA) FHUA_ECOLI 3 3 0 0 0 0 +
61 30S ribosomal protein S2 (rpsB) RS2_ECO27 2 5 0 0 0 0 +
62 Uncharacterized protein YggE (yggE) YGGE_ECO57 2 1 0 0 0 0
63 Inhibitor of g-type lysozyme (pliG) PLIG_ECOLI 2 3 0 0 0 0
64 50S ribosomal protein L13 (rplM) RL13_ECO24 2 1 0 0 0 0 +
65 DNA-directed RNA polymerase subunit beta (rpoB) RPOB_ECOL5 1 4 0 0 0 0 +
66 50S ribosomal protein L5 (rplE) RL5_ECO24 1 4 0 0 0 0 +
a

Live-exo (Live), HI-exo (HI), and none-exo (None) (n = 2) were analyzed by mass spectrometry. The number represent total spectrum count. The proteins reported to be present in OMVs (ref. 15) were indicated by “+” in TABLE 1.