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. 2023 Jan 10;14(1):e03136-22. doi: 10.1128/mbio.03136-22

FIG 2.

FIG 2

Virulence of SARS-CoV mutants with the PBM of E protein from attenuated or virulent β-hCoVs. (A) E protein sequence of parental and the corresponding mutants in which the PBM of E protein was replaced by that of an alternative virulent or attenuated β-hCoV (in red). Groups of five 16-week-old BALB/c mice were inoculated intranasally with DMEM (uninfected, gray), or with 100,000 PFU of parental SARS-CoV (PBM-SARS2), or with the generated mutants: PBM-MERS (orange line), PBM-OC43 (green line), and PBM-HKU1 (dark blue line), and PBM− (pink line). (B and C) Weight loss (B) and survival (C) were monitored for 10 dpi. Vertical bars represent the standard error of the mean weight of the mice. TMD, transmembrane domain.