TABLE 1.
PCR primers used to engineer SARS-CoV-E-PBM mutants
| Virus/primer | Sequence (5′→3′) |
|---|---|
| PBM-MERS | |
| SARS-E-VS | CTCTTCAGGAGTTGCTAATCCAGCAATGG |
| SARS-E-PBM-MERS-RS | TTAGAACCATTCATCAGGAACTCCTTCAGAAGAGTTCAG |
| SARS-E-PBM-MERS-VS | TCTGAAGGAGTTCCTGATGAATGGTTCTAAACGAACTAACTATTA |
| SARS-26885-RS | GGTCCTTAATGTCACAGCGCCC |
| PBM-HKU1 | |
| SARS-E-VS | CTCTTCAGGAGTTGCTAATCCAGCAATGG |
| SARS-E-PBM-HKU1-RS | CGTTTAGATCAGATCATCAGGAACTCCTTCAGAAGAGTT |
| SARS-E-PBM-HKU1-VS | GAAGGAGTTCCTGATGATCTGATCTAAACGAACTAACTATTA |
| SARS-26885-RS | GGTCCTTAATGTCACAGCGCCC |
| PBM-OC43 | |
| SARS-E-VS | CTCTTCAGGAGTTGCTAATCCAGCAATGG |
| SARS-E-PBM-OC43-RS | TTAGACGTCATCAACAGGAACTCCTTCAGAAGAG |
| SARS-E-PBM-OC43-VS | TCTGAAGGAGTTCCTGTTGATGACGTCTAAACGAACTAACTATTA |
| SARS-26885-RS | GGTCCTTAATGTCACAGCGCCC |
| MERS-CoV PBM− | |
| SA27502VS | GCTTATCGTTTAAGCAGCTC |
| Rs mutPBM | CATATTAGACATTATGAAGGAGTTCGTTATCCTCCTCCTCCAGGTGGT |
| VS mutPBM | AACCCCCTCTACCACCTGGAGGAGGAGGATAACGAACTCCTTCATAAT |
| SA28319RS | TCTGTCGTAGTCACAAGCAC |