FIG 7.

Deletion of HCF-1 reduces the number of reactivating neurons. (A and B) Latently infected ganglia from the indicated strain treated with vehicle or tamoxifen were explanted for 48 h in the absence or presence of ACV. Sections were stained with anti-UL29 (red) and DAPI (blue). (A) Sections were scored for UL29⁺ clusters (Clusters) and individual neurons (Single). (n ≥ 6 ganglia per strain/condition; Mann-Whitney test). (B) Representative sections from HCF-1cKO/AvCreERT2 mice. (C) Latently infected mice were treated with vehicle or tamoxifen and subsequently treated with vehicle or JQ1. Ganglia were removed and fixed, and sections were stained with anti-UL29. The number of UL29 (+) neurons were counted (3 sections each of ≥11 ganglia per group; Mann-Whitney test).