TABLE 2.
Oligonucleotides used for genome editing
| Gene | Species | Guide | Sequence (5′–3′) | Exon targeted |
|---|---|---|---|---|
| LRP6 | Canis lupus familiaris | sgRNA1 | GCCACCATTATCAATTCCACA | 5 |
| sgRNA2 | GCAAGGCTCAATGGTACCATG | 6 | ||
| sgRNA3 | GCCCGGACATTCCGAAGACTG | 13 | ||
| Chlorocebus sabaeus | sgRNA1 | GTTGCCTTAGATCCTTCAAGT | 2 | |
| sgRNA2 | GCAAGGCTCAATGGGACCATG | 6 | ||
| STT3A | Canis lupus familiaris | sgRNA1 | GACAGATCGAGAGATATACCC | 6 |
| sgRNA2 | GCTGCGCAGATAATCCACAA | 9 | ||
| Chlorocebus sabaeus | sgRNA1 | GACAGACATTCCGAATGTCGA | 5 | |
| sgRNA2 | GCTGCGCAGGTAATCCACAA | 9 | ||
| KCNE5 | Canis lupus familiaris | sgRNA1 | GCCGCAATCTCGTCGACGTCA | 1 |
| sgRNA2 | GCTGCTTGAGCTGCACCATCG | 1 | ||
| ALCAM | sgRNA1 | GATCCAGACGGCAACATCACA | 5 | |
| sgRNA2 | GATTTGTCCTTAAACCCAAG | 9 | ||
| Non targeting gRNA (NT) | GTGATTGGGGGTCGTTCGCCA | No gene targeted | ||