. 2023 Jan 16;14(1):e03114-22. doi: 10.1128/mbio.03114-22

TABLE 3.

PCR primers used for TIDE analysis

Gene	Species	Guide	Primer	Sequence (5´-3´)	T_m (°C) for PCR protocol
LRP6	Canis lupus familiaris	sgRNA1	Forward	AGGACCTGGATGACATTTGGT	63°C
		sgRNA1	Reverse	GCCCTAAAACACAAAACACCC
		sgRNA2	Forward	AGCACGAAGGACAGACTTGA
		sgRNA2	Reverse	GCCACTGAAGAGCTGTTTACT
		sgRNA3	Forward	TGAGGAGAGTTTCTGGGGAC
		sgRNA3	Reverse	AGGGTGGTTGGTTACTGTAATT
	Chlorocebus sabaeus	sgRNA1	Forward	TGTAGTTGGAGGCTTGGAGG	64°C
		sgRNA1	Reverse	AGGGTGGTGTATGTCAGTGG	64°C
		sgRNA2	Forward	AGGTGCCACAGAATTATTGCT	63°C
		sgRNA2	Reverse	AGAGCTTCTTACCCAACCATG	63°C
STT3A	Canis lupus familiaris	sgRNA1	Forward	AGTCTTGGTATCATCATGGGGA	63°C
		sgRNA1	Reverse	TGGAGGAGATGACAGAGAAAGA	63°C
		sgRNA2	Forward	TTGGCCCTAATTCCTCGGAG	64°C
		sgRNA2	Reverse	CTGGGCTGACAAGTGGACTA	64°C
	Chlorocebus sabaeus	sgRNA1	Forward	AGAGTGGAGATGGGTGGTTT	64°C
		sgRNA1	Reverse	CCACCCTCCAGCATCGATTA	64°C
		sgRNA2	Forward	ACCTGAGCAAAAGCCAGAGA	64°C
		sgRNA2	Reverse	GAGCCTTCCCTACCTGTCAG	64°C
KCNE5	Canis lupus familiaris	sgRNA1	Forward	GGCTACGCACTCTCCTCAAC	64°C
		sgRNA1	Reverse	CTGGAAGGGGAGAGAAGGAG
		sgRNA2	Forward	CTCTGTCTCTCTCTCGCCTG
		sgRNA2	Reverse	AGGCAGGCGTAGAAGATCAT
ALCAM		sgRNA1	Forward	GTCCACAGTCAATGCTTGGG	64°C
		sgRNA1	Reverse	CTGCTCTTGATTCCTCTGCAC	64°C
		sgRNA2	Forward	TGACCATTTCCTTGTCCAAACT	63°C
		sgRNA2	Reverse	AAAGAGAGCAGAGGTGTGGG	63°C