TABLE 2.
Oligonucleotides used in this study
| Primer name | Sequence (5′–3′) |
|---|---|
| Construction of the ΔravA-viaA mutant | |
| ravAwanner_up | CTCGCAATTTACGCAGAACTTTTGACGAAAGGACGCCACTTCATTTGTGTAGGCTGGAGC |
| viaAwanner_do | GCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATCATATGAATATCCTCC |
| Construction of the pRV plasmid | |
| ravA UP BamHI | GGCCGGATCCATGGCTCACCCTCATTTATTA |
| viaA DO HindIII | GGCCAAGCTTTTATCGCCGCCAGCGTCTGAG |
| Checking the knockout mutants | |
| fdhD_Frd | TTTCTTTGCGGAAGGGGCCG |
| fdhD_Rv | GAAAACGCCACTACACGCATTT |
| fdhF_Frd | CGAATGGATAAAAAAACAGCCTCCG |
| fdhF_Rv | ATGACCCCACATAAAATGTGGC |
| frdA_Frd | GTGGAATAGCGTTCGCAGACC |
| frdA_Rv | GCTATGCGGTGCGGTATCGAC |
| glpA_Frd | ATGAGCGAATATGCGCGAAATCAAA |
| glpA_Rv | GCAGTTGCAGGCCACAGAGTAA |
| hyaA_Frd | ATGGTTTGCCTTGCTACAGGGA |
| hyaA_Rv | GCGGCGTCCGGCATTATTG |
| hyaB_Frd | GACCAGCGCAGACGTCATAAC |
| hyaB_Rv | GTGGCTGACAACGTTGTCGC |
| hybC_Frd | CCGATGGCTTCATCGGTCAG |
| hybC_Rv | CGTACTCATTCGTCTACTGCCG |
| hycE_Frd | ATAAGACGAGGTCGCCGTGC |
| hycE_Rv | TATTACTCCGCGCATTACCTGGG |
| menA_Frd | AACATCTGGATGCGTTGGTGG |
| menA_Rv | TAGGCTTAACATTCAGTTGCTGC |
| narG_frd | AGGCTCCCACAGGAGAAAACCG |
| narG_Rv | CACCATGCCGACTTGTGAACGAATTT |
| nuoC_Frd_2 | TGCTCGATCGCTTCACGCTC |
| nuoC_Rv_2 | TCGGCAAAGGGATTTTTCTTCGC |
| ravA_up_verif | CCTAAATGCGGCCACATTAACC |
| viaA_do_verif | GGCGGCGGTATCGCCCAGTCTCG |