TABLE 1.
Immunogenetics of Env-specific monoclonal antibodies from SCIV-infected rhesus macaques
| RM | Expanded lineage | No of Members | Wks post infection | HDCR3 length (AA) | IGHV genea | % HV somatic hypermutation | IGHD genea | IGHJ genea | IGLV geneb | IGLJ geneb | Autologous neutralization | Heterologous neutralization |
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HIV-1 | SIVcpz | ||||||||||||
| T927 | L1 | 3 | 32 | 24 | IGHV4-79*02_S9501 | 5.1 | IGHD3-9*01 | IGHJ6-6*01 | IGLV3-40*01 | IGLJ2A*01 | Yes | 0/19 | 0/1 |
| L2 | 19 | 24 | 15 | IGHV5-15*01_S2502 | 4.2 to 5.8 | IGHD6-13*01 | IGHJ1*02 | IGLV2S9*01 | IGLJ1*01 | Yes | 1/19 | 0/1 | |
| L3 | 3 | 62 | 27 | IGHV4-149*01_S1992 | 5.1 to 6.5 | IGHD3-9*01 | IGHJ5-5*01 | IGLV1-85*01 | IGLJ2A*01 | No | 2/19 | 0/1 | |
| L4 | 9 | 62 | 27 | IGHV4-149*01_S1992 | 6.9 to 9.0 | IGHD3-9*01 | IGHJ4-3*01 | IGLV1-67*02 | IGLJ3*01 | Yes | 5/19 | 1/1 | |
| L5 | 7 | 24, 62 | 22 | IGHV3-50*01 | 4.4 to 14.2 | IGHD3-9*01 | IGHJ5-4*03 | IGKV2-104*02 | IGKJ1*01 | Yes | 6/19 | 1/1 | |
| T925 | L1 | 5 | 24 | 18 | IGHV4-117*01_S5847 | 1.4 to 4.4 | IGHD3-9*01 | IGHJ4-3*01 | IGKV1-94*01 | IGKJ4*01 | Yes | 7/19 | 1/1 |
| L2 | 27 | 24 | 18 | IGHV4-NL_33*01_S4467 | 4.0 to 7.7 | IGHD3-18*01 | IGHJ6-6*01 | IGLV2-23*02 | IGLJ3*01 | No | 0/2 | n.d.c | |
a
Rhesus macaque heavy (H) chain variable (V), diversity (D), and joining (J) gene names are from Bernat et al., 2021 (57), except for the D genes of T927 L1-5 and T925 L1, and the J gene of T927 L2, which are from the International ImMunoGeneTics Information System (IMGT) (see Table S2E at https://doi.org/10.6084/m9.figshare.21644675 for the GenBank accession numbers of the corresponding nucleotide sequences).
b
Rhesus macaque light (L) chain variable (V) and joining (J) gene names are from IMGT.
c
n.d., not done.