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Generation of ΔgB/gB3A virus. Vero cells expressing gB3A under the native viral promoter (Vero-gB3A cells) were transfected with an HSV-1 BAC deleted for the gB gene (pQF282). After 3 weeks at 37°C, cells were imaged at ×200 magnification for GFP (left) encoded by the gB3A expression plasmid and RFP (right) encoded by the virus. The scale bar represents 100 μm.
