FIG 6.

Impact of mutant gH expression on growth of mutant viruses prior to passage. Vero cells were transfected with plasmids encoding gH-S830N or gH-H789Y and then infected with gB3A or HSV-SaHVgB viruses, respectively. Cells transfected with empty vector were infected in parallel. (A) Cells were stained with Giemsa 3 days postinfection. (B) Plaque sizes are expressed as a percentage of plaques sizes on cells transfected with vector. The error bars represent standard deviations. Asterisks indicate a significant difference in plaque size between the Vero cells transfected with gH versus vector (Mann-Whitney U test, P ≤ 0.01).