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. Author manuscript; available in PMC: 2023 Feb 28.
Published in final edited form as: J Vis Exp. 2020 Jul 9;(161):10.3791/61623. doi: 10.3791/61623

Video 1: Animation of the full, 20-minute Aplysia escape locomotor program.

The opacity of the white shapes overlying 81 individual neurons in the right pedal ganglion (left panel) was driven by the corresponding neuronal traces (right panel) and varied linearly as a function of average spike rate (binned per every 0.61 s of real time in the recording). For each neuron, full opacity was normalized to its maximum firing rate over the duration of the recording. One second of elapsed time in the video represents 12.2 s of real time. The scale bar corresponds to real time, with the green, cyan, and dark blue lines below the traces indicating the pre-stimulus baseline, gallop, and crawl phases of the escape locomotor program, respectively. The yellow boxes around the gallop phase and a portion of the crawl phase indicate the recording excerpts used to generate the animations in Videos 2 and 3.