FIGURE 5.

Detection of Human TILs in huNBSGW PDX tumor sections. A, Tumor sections from huPDX were labeled for human T-cell markers CD3, CD4, and CD8. Shown are scanned images of labeled sections at 20× magnification. Representative images are shown for huPDX3 and huPDX18. For huPDX9, the image shown is a rare group of TILs in a mostly negative section to show that the rare T cells were being labeled. Scale bar, 100 μm. B, Quantification of the number of TILs using HALO software. Values are reported as no. of positive cells/100,000 cells. The box plots indicate the mean, minimum, and maximum. Black dots indicate values for each tumor analyzed (n = 2–3).