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. 2023 Feb 28;296:122071. doi: 10.1016/j.biomaterials.2023.122071

Fig. 2.

Fig. 2

Infilling of cholesterol decreases the membrane permeability of NVs to establish a pH gradient between intra-vesicles and outer-vesicles. (A) Cryo-TEM image of NVs. (B) pH values in NVs measured using a pH indicator of SNARF-1 entrapped inside NVs and NVs were suspended in PBS at pH7.4; (C) Fluorescent intensity of SNARF-1 contained in NVs after the incorporation of cholesterol to NVs; (D) Fluorescent intensity of fluorescein sodium contained in NVs after the incorporation of cholesterol to NVs; (E) Cholesterol percentages in NVs determined using a cholesterol fluorometric assay kit; (F) Sizes of NVs with or without addition of cholesterol; (G) Zeta potentials of NVs with or without addition of cholesterol; In E, F and G, 10% (w/w) cholesterol was supplemented. Data presented as the mean ± SD, n = 3, 6. **P < 0.01, ***P < 0.001.