Figure 3.

Evaluation of CD56^brightCD16^- NK_reg cell ability to suppress CD4⁺ T-cell proliferation. Suppressive effect of (A) CD56^brightCD16^- regulatory NK (NK_reg) cells, (B) CD56^dimCD16⁺ NK cells, and (C) T_reg cells towards allogeneic CD4⁺ T cells. On the histograms, the x-axis displays the cell proliferation dye (CPD) eF450 and the y-axis displays the cell count. (D) Suppressive capacity of CD56^brightCD16^- NK_reg cells, CD56^dimCD16⁺ NK cells, and T_reg cells towards allogeneic CD4⁺ T cells at the 1:1, 1:2, 1:4, and 1:8 ratios as compared to the activated CD4⁺ T-cell control (mean +/- standard deviation). *P<0.05, ***P<0.0005. Statistical analyses for suppression experiments were performed using Microsoft Excel version 2110 and a two-tailed t test – two-sample assuming unequal variance. The data is from a single experiment representative of 5 experiments for the NK-cell vs. CD4⁺ T-cell assays, and 2 experiments for the T_reg cell vs. CD4⁺ T-cell assay.