Figure 4.

Evaluation of CD56^brightCD16^- NK_reg cell ability to suppress CD8⁺ T-cell proliferation. Suppressive effect of (A) CD56^brightCD16^- regulatory NK (NK_reg) cells towards allogeneic CD8⁺ T cells compared to the CD8⁺ T-cell control. On the histograms, the x-axis displays the cell proliferation dye (CPD) eF450 and the y-axis displays the cell count. (B) Proliferation of CD8⁺ or CD4⁺ T cells after 96-hour co-culture with allogeneic CD56^brightCD16^- NK_reg cells at the 1:1 ratio as compared to the activated CD8⁺ or CD4⁺ T-cell controls (mean +/- standard deviation). *P<0.05, ***P<0.0005. Statistical analyses for suppression experiments were performed using Microsoft Excel version 2110 and a two-tailed t test – two-sample assuming unequal variance. The data is from a single experiment representative of 3 experiments.