Figure 6.

Evaluation of cell-cell contact dependence for CD56^brightCD16^- NK_reg cell suppression. Suppressive effect of CD56^brightCD16^-relulatory NK (NK_reg) cells towards allogeneic CD4⁺ T cells when cells are co-cultured for 96 hours at the ratio 1:1 in a standard round-bottom 96-well plate or 96-well transwell plate. (A) Proliferation of CD4⁺ T cells (control), (B) proliferation of CD4⁺ T cells co-cultured with NK_reg cells in a standard plate, (C) proliferation of CD4⁺ T cells co-cultured with NK_reg cells in a transwell plate. On the histograms, the x-axis displays the cell proliferation dye (CPD) eF450 and the y-axis displays the cell count. (D) Proliferation of CD4⁺ T-cell control compared to CD4⁺ T cells co-cultured with NK_reg cells in a standard or transwell plate (mean +/- standard deviation). ***P<0.0005, NS: not significant. Statistical analyses were performed using Microsoft Excel version 2110 and a two-tailed t test – two-sample assuming unequal variance. The data is from a single experiment representative of 5 experiments.