FIG. 1.

Physical structures of plasmids. The inserted DNA fragments are indicated by lines. A 5.5-kb XhoI fragment of pRH71 is indicated by a heavy line. The region sequenced is indicated. The predicted ORFs are shown as open arrows and an open box, which indicates a 3′-truncated gene. It is thought that in pES1-23a ORF 1 is expressed as a lacZ-fused protein (open box plus an open arrow under the lac promoter). The positions of ORF 2 and ORF 3 in pES1-23a are not shown.