TABLE 1.

Results of screening for microbial proline 4-hydroxylases

Strain	Proline 4-hydroxylase activity		Etamycin production
	Cellular activity (U/g of cells)	Sp act (U/mg of protein)
S. griseoviridus P-8648	22.0	1.63	+
S. griseoviridus JCM 4250	20.0	0.86	+
S. daghestanicus JCM 4365	9.0	0.14	+
Dactylosporangium sp. strain RH1	28.0	2.31	−
Dactylosporangium sp. strain RH3	21.5	0.22	−
Dactylosporangium sp. strain RH4	4.0	NDa	−
Amycolatopsis sp. strain RH2	6.9	ND	−
Amycolatopsis sp. strain RH5	3.0	ND	−

^aND, not determined.