Table 2. Characteristics of armadillo tissue samples from US museums identified as positive by standard PCR for Mycobacterium leprae*.
Voucher/tissue no. | Sample ID | Tissue type | Preservation (%) | DNA con. | Country | State | Sex | Year | Type | Subtype | Ct |
---|---|---|---|---|---|---|---|---|---|---|---|
YPM 16952 | 63 | Muscle | Ethanol (95) | 20 | USA | Texas | F | 2014 | ND | ND | ND |
YPM 15982 | 66 | Muscle | Ethanol (95) | 13 | USA | Texas | F | 2015 | ND | ND | ND |
YPM 15294 | 80 | Muscle | Ethanol (95) | 2.89 | USA | Texas | F | 2013 | 3 | 3I | 34.41 |
YPM 16954 | 95 | Muscle | Ethanol (95) | 11 | USA | Texas | M | 2014 | ND | ND | ND |
YPM 15295 | 97 | Muscle | Ethanol (95) | 14 | USA | Texas | F | 2013 | ND | ND | ND |
YPM 15292 | 99 | Muscle | Ethanol (95) | 5.7 | USA | Texas | F | 2013 | ND | ND | ND |
YPM 15296 | 103 | Muscle | Ethanol (95) | 8.9 | USA | Texas | F | 2013 | ND | ND | ND |
YPM 15293 | 105 | Muscle | Ethanol (95) | 4.76 | USA | Texas | M | 2013 | ND | ND | ND |
YPM 14944 | 109 | Muscle | Ethanol (95) | 9.3 | USA | Texas | NA | 2014 | 3 | 3I | 23.15 |
YPM 15315 | 110 | Muscle | Ethanol (95) | 0.0041 | USA | Texas | F | 2013 | ND | ND | ND |
YPM 15298 | 111 | Muscle | Ethanol (95) | 27 | USA | Texas | F | 2013 | ND | ND | ND |
YPM 15299 | 115 | Muscle | Ethanol (95) | 43 | USA | Texas | F | 2012 | ND | ND | ND |
UAM 46589 | 118 | Liver | DMSO | 11 | Paraguay | Canindeyu | F | 1996 | ND | ND | ND |
MSB 140243 | 138 | Liver | Ethanol (95) | 37 | Bolivia | Beni | NA | 1993 | ND | ND | ND |
TTU 75235 | 158 | Spleen | Frozen | 19 | USA | Texas | F | 1996 | 3 or 4 | ND | 35.12 |
TTU 82457 | 194 | Muscle | Frozen | 3.82 | USA | Texas | M | 2000 | 3 | 3I | 31.58 |
TTU 75360 | 209 | Spleen | Frozen | 20 | USA | Texas | F | 1996 | 3 | 3I | 25.83 |
TTU 80673 | 212 | Spleen | Frozen | 31 | USA | Texas | M | 1999 | ND | ND | ND |
*We identified a total of 18 M. leprae-positive samples. Bold text indicates samples suitable for whole-genome sequencing (n = 2). Samples negative for subtyping were determined unsuitable for whole-genome sequencing. Ct determined by quantitative PCR. Ct, cycle threshold; DNA con., concentration of total DNA per sample; NA, no data available; ND, not determined