Table 1.
MSD Platform Analyte Detectable Range and Quality Control
| Panel | Catalog Number | Analyte | Calculated Min Detectable Concentration (AU/mL) | Calibrator Range (AU/mL) | Dilution Factor | Detectable Assay Range (AU/mL) | QC CV% Range | QC CV% Average | Comment | |
|---|---|---|---|---|---|---|---|---|---|---|
| Coronavirus Serology | K15369U | Spike | 0.0035 | 3.52 | 0.004272 – 700.00 | 1,000 | 4.272 – 700,000 | 1.63% – 12.46% | 6.08% | Quantitative IgG Serology: Calibrator in Arbitrary Units/mL (AU/mL) |
| RBD | 0.0024 | 2.36 | 0.001831 – 300.00 | 1,000 | 2.36 – 300,000 | 5.14% – 55.36% * | 22.09% | |||
| Nucleocapsid | 0.0058 | 5.75 | 0.004883 – 800.00 | 1,000 | 5.75 – 800,000 | 0.06% – 6.83% | 3.05% | |||
| 229E | 0.0027 | 2.73 | 0.0018 – 300.00 | 1,000 | 1.83 – 300,000 | 0.03% – 7.16% | 2.79% | |||
| CoV-1 Spike | 0.0049 | 4.91 | 0.0003 – 50.00 | 1,000 | 4.91 – 50,000 | 2.15% – 26.21% * | 8.77% | |||
| CoV-2 NTD | 0.00018 | 0.18 | 0.000061 – 10.00 | 1,000 | 0.18 – 10,000 | 1.21% – 62.51% * | 16.00% | |||
| HKU1 | 0.0965 | 96.47 | 0.0018 – 300.00 | 1,000 | 96.47 – 300,000 | 2.39% – 14.65% | 6.39% | |||
| NL63 | 0.0031 | 3.08 | 0.0003 – 50.00 | 1,000 | 3.08 – 50,000 | 2.56% – 8.86% | 4.91% | |||
| OC43 | 0.1010 | 101.04 | 0.0031 – 500.00 | 1,000 | 101.04 – 500,000 | 1.52% – 7.16% | 4.49% | |||
Validation data was performed by the manufacturers of the MesoScale Discovery (MSD) Platform. Validation data performed by the authors included comparison of the data collected from the MSD against other methods, including the Luminex SARS-CoV-2 panel, Quanterix Spike Assay, UVM Homebrew Assay(s), and some ELISAs, to ensure accuracy and reproducibility. Other validation data performed by the authors to ensure reproducibility and validity of study data include PCR-positive/negative agreement, precision and accuracy, and lot-to-lot validation.
*
High CV% Driven by pre-pandemic QC replicate(s); replicates low on calibrator curve