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. 1999 Sep;65(9):4032–4039. doi: 10.1128/aem.65.9.4032-4039.1999

FIG. 2.

FIG. 2

Agarose gel electrophoresis of BamHI-XhoI-EcoRI-digested plasmid DNA from native and transformed B. thuringiensis Kto and Kto SigK− strains. Lanes: 1 and 8, 1-kb DNA ladder; 2, Kto recipient; 3, Kto (pHTF3-1C/Ab-IRS-T-Δ); 4 and 7, pHTF3-1C/Ab-IRS-T isolated from E. coli; 5, Kto SigK− (pHTF3-1C/Ab-IRS-T-Δ); 6, Kto SigK− recipient. The 2.9-kbp BamHI DNA fragment corresponding to the pBluescript II KS(−) and the 1.6-kbp BamHI-EcoRI DNA fragment corresponding to the tet gene of B. cereus are indicated by black arrows (lane 7). These DNA fragments are absent from the Kto and Kto SigK− transformants harboring pHTF3-1C/Ab-IRS-T-Δ. Only the 2.6-kbp EcoRI-XhoI and 4.3-kbp BamHI-EcoRI DNA fragments corresponding to the origin of replication of pHT1030 and the cry1C/Ab gene, respectively, remain (indicated by open triangles in lanes 3 and 5) after the site-specific recombination that removes the tet gene and pBluescript II KS(−). The sizes (in kilobase pairs) of the 1-kb ladder are shown on the right.