TABLE 1.
B. thuringiensis strains and plasmids
| Strain or plasmid | Relevant genotype or plasmid characteristics | Reference(s) |
|---|---|---|
| 407− | Acrystalliferous derivative of the wild-type B. thuringiensis strain 407 (H1 serotype) isolated by O. Arantes | 3, 18 |
| Kto (HD73) | Wild-type B. thuringiensis strain containing Tn4430 and a cry1Ac gene | 15, 31 |
| Kto− | Acrystalliferous derivative of the wild-type B. thuringiensis strain Kto | 31 |
| 407−(pHTF3-1C) | Cry1C δ-endotoxin produced in the 407− background | This report |
| 407−(pHTF3-1C/Ab) | Cry1C/Ab chimeric δ-endotoxin produced in the 407− background | This report |
| AGRO2 | Kto strain electrotransformed with plasmid pHTF3-1C/Ab-IRS-T and containing pHTF3-1C/Ab-IRS-T-Δ as a result of site-specific recombination in vivo | This report |
| Kto SigK− | Kto strain whose sigK gene was disrupted by the insertion (by homologous recombination) into the chromosome of a sigK gene interrupted by the aphA3 kanamycin resistance gene (sigK::aphA3) | This report |
| AGRO1 | Kto SigK− strain containing pHTF3-1C/Ab-IRS-T-Δ as a result of site-specific recombination in vivo after electrotransformation of the host strain with pHTF3-1C/Ab-IRS-T | This report |
| pAB2 | Plasmid containing the sigK gene of B. thuringiensis strain 407 disrupted by the aphA3 gene of E. faecalis, cloned into pRN5101 | 7 |
| pHT81 | Plasmid containing a chimeric cry1C/Ab gene, consisting of the 5′ end region of the cry1C gene fused (at the KpnI site) to the 3′ end region of the cry1Ab gene, cloned in pUC19 | 29 |
| pHTF3-1C | Plasmid consisting of the coding sequence of the cry1C gene fused to the sporulation-independent cry3A promoter inserted into pHT315 (3) | 30 |
| pHTF3-1C/Ab | Plasmid consisting of the coding sequence of the chimeric cry1C/Ab gene fused to the cry3A promoter and inserted into pHT315 (3) | This report |
| pHTBS2 | Plasmid containing the origin of replication of the B. thuringiensis resident plasmid pHT1030 and the tetracycline resistance gene carried by pBC16 of B. cereus cloned into pBluescript II KS(−) | 30 |
| pHT-IRS-BSK | Plasmid consisting of the pBluescript II KS(−), the aphA3 gene of E. faecalis, and one copy of the IRS of Tn4430 | 31 |
| pHTF3-1C/Ab-IRS-K and pHTF3-1C/Ab-IRS-T | Site-specific recombination vectors consisting of the pBluescript II KS(−) and the kanamycin resistance gene aphA3 of E. faecalis (pHTF3-1C/Ab-IRS-K) or the tetracycline resistance gene carried by pBC16 of B. cereus (pHTF3-1C/Ab-IRS-T) between duplicated copies of the IRS of transposon Tn4430 and segregated from the native B. thuringiensis plasmid components: the chimeric cry1C/Ab gene under the control of the cry3A promoter and the replication and stability regions of plasmid pHT1030 | This report |