Extended Data Fig. 3. alternata response is susceptible to inhibition by microbial factors.

A. a, Eosinophils (CD45⁺ SiglecF⁺ CD11b⁺ CD11c⁻ cells) were identified by being in the lung parenchyma or airways by being unlabeled by a fluorescently tagged anti-CD45 antibody injected intravenously three minutes before euthanasia. Shown are B6 mice treated 24 hours prior with intranasal PBS or A. alternata (Alt) or HpARI and Alt. Numbers in plots represent percent of cells in the gate. b, Number of eosinophils in the lungs and airways (i.v. CD45⁻) 24 hours after indicated intranasal treatment. Mice were B6 (white circles) or B6xBALB/c IL-5^WT/venus (IL-5v F1, light blue squares). Bar graph shows mean +SD of log transformed values. c, Representative flow plots of IL-5 venus expression in IL-5v F1 lung ILC2. d, IL-5 venus gMFI of IL-5 venus⁺ lung ILC2. e-f, ST2 (e) and CD25 (f) of B6 and IL-5v F1 mice. gMFIs from multiple experiments normalized to PBS-treated group set to 100. b and e-f, Pooled from one B6 and one IL-5v F1 experiment (n = 5–9/group). d, Data from one experiment in IL-5v F1 mice (n = 2–3/group). Bar graphs show mean +SD. Each symbol represents a mouse. P values were determined with one-way ANOVA with Tukey’s multiple comparisons test; n.s. p ≥ 0.05. Source Data contains exact P-values and group sizes.