Extended Data Fig. 4. ILC2 numbers and CD4⁺ T cell data from two-week cohoused mice.

Flow analysis 24 hours after intranasal PBS or Alt treatment. Mice were B6 (white) or IL-5v F1 (light blue) and were cohoused with pet store mice for approximately two weeks (2wk) or were age-matched SPF controls. a, f, Data in Figures 2a and 2d are presented again, with samples from each experiment indicated with a different symbol/color combination so that experiment-to-experiment variation can be visualized. b, Number of lung ILC2. c-d, IL-5 venus expression within lung (i.v. CD45⁻) CD4⁺ T cells of IL-5v F1 SPF and 2wk CoH mice 24 hours after PBS or Alt treatment. c, Representative flow plots of IL-5 venus expression in lung CD4⁺ T cells. d, Percent IL-5 venus⁺ and e, normalized IL-5 venus gMFI of IL-5 venus⁺ lung CD4⁺ T cells. b, Pooled from four B6 experiments and two IL-5v F1 experiments (n = 12–14/group). c-e, Pooled from two IL-5v F1 experiments (n = 4–6/group). Numbers in plots represent percent of cells in the gate. Bar graphs show mean +SD. g, Mice were treated with intranasal PBS or papain every other day three times and analyzed 24 hours after the last treatment and are from the same experiments described in Fig. 2g–j. Number of lung i.v.⁻ CD4⁺ T cells in 2wk or >2mo CoH B6 mice or age-matched SPF controls. Bar graphs show mean +SD of log-transformed values. Each symbol represents a mouse. P values were determined with a 2-way ANOVA with Tukey’s multiple comparisons test; n.s. p ≥ 0.05. Source Data contains exact P-values and group sizes.