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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Nat Immunol. 2022 Nov 21;23(12):1703–1713. doi: 10.1038/s41590-022-01350-8

Fig. 3. Acute IL-33, IL-5 and IL-13 responses after A. alternata exposure are reduced in two-month cohoused mice.

Fig. 3.

a, IL-33 in the bronchoalveolar lavage fluid (BALF) of SPF and >2mo CoH B6 mice one hour after Alt exposure, detected by ELISA. Pooled from three experiments (n = 3–10/group). b-e, Alt (b-c), recombinant IL-33 (rIL-33, d-e) or control phosphate buffered saline (PBS) were given intranasally to B6 mice and lungs and BAL were collected 4.5 hours later. IL-5 and IL-13 in the lung homogenates were detected by ELISA and normalized to the amount of total protein in the samples. b-c pooled from three experiments (n = 6–10/group). d-e pooled from three experiments (n = 5–12/group). Bar graphs show mean +SD. Each symbol represents a mouse. P values were determined with 2-way ANOVA with Tukey’s multiple comparisons test; n.s. p ≥ 0.05. Source Data contains exact P-values and group sizes.