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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Nat Immunol. 2022 Nov 21;23(12):1703–1713. doi: 10.1038/s41590-022-01350-8

Extended Data Fig. 1. Serology screening for SPF pathogens in cohoused mice.

Extended Data Fig. 1.

Blood samples were collected and tested for antibodies against common murine pathogens. Each column represents an animal. Each row indicates a pathogen. Filled boxes indicate positive results, lighter shaded boxes indicate equivocal (weak positive) results. White boxes indicate negative results. a, Serology results from representative pet store (n = 8), cohoused B6 (n = 26) and F1 IL-5v (n = 11) mice cohoused for approximately two weeks at the time of blood collection. b, Serology results from representative pet store and cohoused B6, F1 IL-5v, and BALB/c mice cohoused for at least two months at the time of blood collection (n = 26/group). CPIL = Clostridium piliforme, ECUN = Encephalitozoon cuniculi, EDIM = rotavirus, LCMV = lymphocytic choriomeningitis virus, MAV1+2 = mouse adenovirus 1 and 2, MCMV = murine cytomegalovirus, MHV = mouse hepatitis virus, MNV = murine norovirus, MPUL = Mycoplasma pulmonis, MPV1+2 = mouse parvovirus type 1 and type 2, MVM = minute virus of mice, POLY = polyoma virus, PVM = pneumonia virus of mice, REO = reovirus, SEND = murine respirovirus (Sendai virus), TCMV = GDVII Theiler’s murine encephalomyelitis virus. c. Representative flow cytometry histograms of protein expression of lung ILC2 in age-matched SPF (blue) and CoH (red) mice at 2wk and >2mo. Vertical gray lines are added to aid in comparison of expression among groups.