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. 2023 Feb 21;2023:2264030. doi: 10.1155/2023/2264030

Figure 4.

Figure 4

Activation of ALDH2 reduced inflammation and inhibited the phosphorylation of IκBα/NF-κB p65 in I/R. (a)–(c) The expression of IL-10β, MCP-1, and TNF-α mRNA in the kidney was determined by real-time PCR. GAPDH was the standard. n = 3/group. (d) The western blot band of p-IκBα, IκBα, p-p65, p65, and IL-17C in kidney lysates. β-Actin was the standard. n = 3/group. (e)–(g) The western blot analysis of p-IκBα, IκBα, p-p65, p65, and IL-17C protein in kidney samples from each group. n = 3/group. Data are presented as the mean ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 by t-test.