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. 2023 Feb 21;2023:2264030. doi: 10.1155/2023/2264030

Figure 6.

Figure 6

NF-κB phosphorylase inhibitors attenuate the aggravation of injury caused by ALDH2 deficiency. (a) The western blot analysis of ALDH2 in HK-2 lysates. β-Actin was the standard. n = 3/group. (b) Kidney lysates from WT mice were subjected to western blot analysis for ALDH2. n = 3/group. (c) Cell lysates from HK-2 cell after knockdown ALDH2 and JSH-23 preconditioning were subjected to enzyme assay for ALDH2. n = 3/group. (d) Schematic diagram of HK-2 cell treatment. (e) The apoptosis of HK-2 cells was measured by flow cytometry, and the statistical results were expressed by histogram (f). n = 3/group. (g) The western blot band of p-IκBα, IκBα, p-p65, p65, and IL-17C in kidney lysates. β-Actin was the standard. n = 3/group. (h)–(j) Western blot analysis for p-IκBα, IκBα, p-p65, p65, and IL-17C protein in HK-2 cell samples from each group. n = 3/group. Data are presented as the mean ± SD. n.s.: not significant. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 by t-test.