(A) PCR amplification of a putative HMG-box sequence from mating type testers of the G. fujikuroi species complex with degenerate primers NcHMG1 and NcHMG2. Lane 1, N. crassa FGSC 4066; lane 2, λ phage DNA digested by PstI; lanes 3 through 16, G. fujikuroi A-00149, A-00999, B-03852, B-03853, C-01993, C-01995, D-04853, D-04854, E-00990, E-02192, F-04093, F-04094, G-05111, and G-05112, respectively. (B) PCR amplification of the HMG box with Gibberella-specific primers GfHMG1 and GfHMG2. Lanes 2 to 16 are amplification products from the same source as in panel A. Lane 1, HMG-box sequence amplified from G. fujikuroi A-00999 by PCR with the NcHMG1 and NcHMG2 primers.