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. 2022 Dec 31;64(3):100326. doi: 10.1016/j.jlr.2022.100326

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — The mRNA expression levels of fatty acid metabolism-related genes in liver of WT and Ehhadh transgenic zebrafish (Tg:Ehhadh). A: Effects of Ehhadh overexpression on mRNA expression of PUFA synthesis-related genes in liver. B: Effects of Ehhadh overexpression on mRNA expression of Peroxisome β-oxidation related genes in liver. C: Effects of Ehhadh overexpression on mRNA expression of pparα and srebf1 in liver. D: The effect of Ehhadh overexpression on DHA synthesis pathway. The blue arrow indicates the downward adjustment, and the red arrow indicates the upward adjustment. ns means no significant difference; ∗ means P < 0.05; ∗∗ means P < 0.01; ∗∗∗ means P < 0.001. acaa1, acetyl-CoA acyltransferase 1; acaa2, acetyl-CoA acyltransferase 2; acox1, acyl-CoA oxidase 1; acox3, acyl-CoA oxidase 1; Ehhadh, enoyl-CoA hydratase/3-hydroxyacyl CoA dehydrogenase; elovl2, fatty acid elongase 2; elovl4a, fatty acid elongase 4a; elovl4b, fatty acid elongase 4b; elovl5, fatty acid elongase 5; fads2, fatty acid desaturase 2; hsd17b4, hydroxysteroid 17-beta dehydrogenase 4; pparαa, peroxisome proliferator activated receptor alpha a; pparαb, peroxisome proliferator activated receptor alpha b; srebf1, sterol regulatory element binding transcription factor 1.