Fig. 1. NO production temporally correlates with the loss of catalytically active lipoic arm and PDHC and OGDC activity.
a, PDHC and OGDC activity, DLAT and DLST level and their lipoylation state, and iNOS level in RAW 264.7 cells stimulated with LPS + IFN-γ for the indicated time. b, PDHC activity, iNOS expression, protein lipoylation state and total DLAT level in murine BMDM stimulated with LPS + IFN-γ for the indicated time. c–e. The abundance of intracellular acetyl-CoA (c), succinyl-CoA (d) and citrulline (e) in BMDM stimulated with LPS + IFN-γ for the indicated time. ND, not detected. All bars and error bars represent mean ± s.d. (n = 3 distinct samples). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. Bars not sharing a letter/number are significantly different (P < 0.05) from one another. Exact P values for each comparison are available in the source data. a,b, On lipoic arm western blots, specific bands corresponding to the lipoic arm of DLAT and DLST are labeled based on the expected molecular mass and the position of DLAT and DLST bands, as determined by total DLAT/DLST blots. The blots shown are representative of at least two independent experiments.