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FISH verification for dot chromosomes. (A) The karyotype of a chicken cell. (B–F) The probes of nine dot chromosomes bind to different chromosomes in the same cell. The probes are composed of unique oligonucleotides (Dataset S2) and chromosome-specific amplicons (Fig. 5F). Chromosome 16 has been verified by previous studies (27). The scale for the white bar: 10 μm.
