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. 2023 Feb 13;120(8):e2216641120. doi: 10.1073/pnas.2216641120

Fig. 5.

Fig. 5.

The expansion of PCH and amplicons. (A) The dot-chromosomes have larger PCH and the proportion of PCH is higher relative to microchromosomes and macrochromosomes. (B) Chicken chr12 and chr29 are homologous with amphioxus chr4. Six ohnolog pairs between chr29 and chr12 are highlighted. The PCH region of chr29 is rich in TEs while the non-PCH region is depleted of retrotransposons but is enriched for the PO41 repeats (37). (C) Satellite DNA is the most abundant repeat sequence on dot-chromosomes. (D) In macrochromosome and microchromosomes the relative H3K9me3 levels are similar for LINEs and satellite DNA while in dot-chromosomes the satellite DNA tends to have a lower relative H3K9me3 level (red dashed ellipse). (E) The PCH of dot chromosomes contains a large number of multicopy genes. Each green vertical bar represents one copy. (F) OR29G was detected in the PCH of chr29 while the synthesized oligonucleotides (oligo-chr29) were detected in the other part of chr29. (G) The expression of multicopy genes is testis-biased for seven out of the 10 dot chromosomes.