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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Liver Int. 2022 Nov 21;43(3):673–683. doi: 10.1111/liv.15476

Figure 4. Ammonia induced desynchrony among single cells in adult SCN which was prevented by AMPA receptors blocking.

Figure 4.

Representative single cell traces of PER2LUC expression in VEH (A, n=6), ammonia 5 mM (B, n=6) and ammonia 5mM + GYKI 100 μM (C, n=4) with their respective raster plots (A’, B’ and C’) depicting all cells from a representative single SCN explant showing desynchrony induced by ammonia that was prevented by GIKI application. (D) Ensemble mean±SEM PER2LUC expression traces from the same single SCN explants. (E) Group mean±SEM sync indices showing that high synchrony among SCN cells was re-stablished when AMPA receptors were blocked with GYKI. (F) Ammonia increased period (lower panel, *p>0.001) of single SCN cells which was correlated with an increase in the coefficient of variation (top panel, *p=0.03).