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. 2023 Jan 20;415(8):1497–1504. doi: 10.1007/s00216-023-04545-6

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© Crown 2023, corrected publication 2023

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Fig. 2 — UHPLC-Q-Exactive-MS analysis and representative MS/MS spectrum of modified peptide of DTNLC_(ATZ)AIHAK peptide (a) and unmodified DTNLCAIHAK peptide (b) from human histone H3.3. The MS/MS spectrums are that of the double-charged ion at m/z 632.833 and 543.275, corresponding to DTNLC_(ATZ)AIHAK and DTNLCAIHAK peptide. This covalent modification was identified under adduction assay conditions of human histone H3.3 (1 mg/mL H3.3 in PBS buffer) incubated with 20 µg/mL of atrazine for 24 h and then digested by trypsin/Glu-C