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. 2023 Jan 20;415(8):1497–1504. doi: 10.1007/s00216-023-04545-6

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Fig. 4 — UHPLC-Q-Exactive-MS analysis and the profile of concentration-dependent adduct formation of calf thymus histone and atrazine. The peak area ratio is of the peak area in extracted ion chromatogram (EIC) of m/z 632.833 versus that of m/z 543.274. The incubation conditions were calf thymus histone solutions (1 mg/mL histone in PBS buffer) incubated with different atrazine concentrations (0 to 20 μg/mL), and at 37 °C for 24 h, and then digested by trypsin/Glu-C. All incubations were carried out in triplicate