Fig. 3.

BRD4 and PI3K dual inhibitor SF2523 and its novel analogs MDP1 and MDP5 inhibit MB cell proliferation and migration in culture. A&B Cell viability assay in DAOY and HD-MB03 cells after incubation with different concentrations of MDP series compounds. C In the Transwell migration assay, cells were treated with SF2523, MDP1, and MDP5 at 5.0 μM concentration for 24 h. Cells were trypsinized and placed in apical chamber of insert in 24 well plate to 24 h. Next, migrated cells were fixed in formalin and stained with 0.5% crystal violet for 30 min. Images were taken using a microscope at 20x. D Next, stained cells were dissolved in 10% acetic acid solution and measured optical density (OD) at 590 nm. Scale bar, 100 μm; ** P < 0.01 and *** P < 0.001 vs. Control.